Ozone can destroy the cell wall of decomposing bacteria, quickly diffuse into the cell, oxidize and decompose glucose oxidase, which is necessary for oxidizing glucose inside bacteria, and can also directly interact with bacteria and viruses to destroy Cells, ribonucleic acid (RNA), break down deoxyribonucleic acid (DNA), RNA, proteins, lipids, polysaccharides and other macromolecular polymers, which destroys the bacteria's metabolism and reproduction process. The killing of bacteria by ozone is caused by the rupture of the cell membrane. This process is called cell dissipation. It is caused by the crushing of cytoplasm in water. Under the condition of dissipation, cells cannot regenerate.
It should be noted that unlike hypochlorous acid disinfectants, ozone’s sterilization ability is not affected by changes in pH and ammonia, and its sterilization ability is 600-3000 times greater than that of chlorine. Its sterilization and disinfection effects occur almost instantaneously. When the ozone concentration in water reaches 0.3-2mg/L, bacteria can be killed within 0.5-1min. The dose of ozone water medicine required to achieve the same sterilization effect is only 0.0048% of chlorine. Common E. coli, Streptococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus, mold, etc., in 15 minutes in the ozone environment, the kill rate can reach 99.999%.